[2017-01-11 10:54:52] Beginning TopHat run (v2.1.1) 
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[2017-01-11 10:54:52] Checking for Bowtie 
Bowtie version: 2.2.9.0 
[2017-01-11 10:54:53] Checking for Bowtie index files (genome).. 
[2017-01-11 10:54:53] Checking for reference FASTA file 
[2017-01-11 10:54:53] Generating SAM header for /Users/SK/my_rna_seq/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome 
[2017-01-11 10:54:53] Reading known junctions from GTF file 
[2017-01-11 10:55:04] Preparing reads 
left reads: min. length=101, max. length=101, 27273582 kept reads (108679 discarded) 
right reads: min. length=101, max. length=101, 27273582 kept reads (108679 discarded) 
[2017-01-11 11:26:08] Building transcriptome data files A549_thout/tmp/genes 
[2017-01-11 11:27:08] Building Bowtie index from genes.fa 
[2017-01-11 11:41:34] Mapping left_kept_reads to transcriptome genes with Bowtie2  
 
[2017-01-11 14:09:27] Mapping right_kept_reads to transcriptome genes with Bowtie2  
[2017-01-11 16:37:02] Resuming TopHat pipeline with unmapped reads 
[2017-01-11 16:37:02] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2  
[FAILED] 
Error running bowtie: 
Out of memory allocating plen[] in Ebwt::read() at bt2_io.cpp:272 
Error: Encountered exception: 'std::bad_alloc' 
Command: /usr/local/bin/../Cellar/bowtie2/2.2.9/bin/bowtie2-align-l --wrapper basic-0 -k 20 -D 15 -R 2 -N 0 -L 20 -i S,1,1.25 --gbar 4 --mp 6,2 --np 1 --rdg 5,3 --rfg 5,3 --score-min C,-14,0 -p 1 --sam-no-hd -x /Users/SK/my_rna_seq/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome -  
(ERR): bowtie2-align exited with value 1   | 
             
           
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