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逆転写で使ったのと同じプライマーでPCRで、増えない トピック削除
No.5590-TOPIC - 2016/12/01 (木) 02:04:30 - RT
不思議な現象に出くわして戸惑っているのですが、ある遺伝子から13塩基ほど下流のプライマー(その遺伝子の3'末端と、3塩基オーバーラップ)で逆転写を行い、その遺伝子の全長(下流の配列を含まない)のプライマーセットでPCRを行ったらバンドが得られたものの、逆転写で使った「13塩基+3塩基」のプライマーでPCRを行ったら、理論上は全長+13塩基のバンドが得られるはずだと思うのですが、何も増えませんでした(Forwardは当然同じプライマーです)。

あまりにも謎すぎてなぜそうなるのか不明なのですが、何か見落としている落とし穴などがありましたらご教示いただけるとありがたく思います。
 
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